Metagenomic analysis has become an indispensable tool for comprehensively profiling microbial communities, revealing not only who is there (taxonomic and ecological structure) but also what they are doing (functional potential).
Once your sequences are generated, the real challenge begins: transforming raw data into actionable biological insights. This process is notoriously complex and computationally intensive.
Whether your project involves shotgun metagenomics (sequencing all genomic material) or metataxonomics (targeting specific marker genes), our experts guide you through the entire analytical journey—from raw data to clear, interpretable results.
With over a decade of experience in high-throughput sequencing (NGS) data analysis, we specialize in unlocking the secrets of complex microbial ecosystems. Our custom bioinformatic pipelines are designed to deliver precise, reproducible results aligned with your specific research goals in metagenomics.
Raw sequencing data often contains low-quality or truncated reads, with quality scores typically decaying toward the ends of each sequence. Contaminants or residual sequencer adapters may also be present. To ensure the integrity of your downstream metagenomic analysis, rigorous quality control and preprocessing are essential first steps.
Once your reads are filtered and quality-checked, the next critical phase is assembly—reconstructing contiguous genomic sequences (contigs) from fragmented data. This stage requires selecting the optimal assembler for your dataset and rigorously evaluating outputs to mitigate artifacts like chimeras. Given its computational intensity, assembly often demands significant processing power. We provide expert metagenomic assembly services, leveraging our experience and resources to accurately reconstruct your microbial communities.
Following assembly, we profile the taxonomic composition of your microbial community using established phylogenetic markers like 16S rRNA or ITS. This step is essential for understanding the “who” in your sample—providing the critical foundation for all downstream ecological and functional interpretation.
Building on your community’s taxonomic profile, we provide a comprehensive ecological assessment. We analyze alpha-, beta-, and gamma-diversity to quantify richness, evenness, and compositional shifts across your samples. Furthermore, we examine how diversity metrics and taxonomic composition change in response to your specific experimental or environmental factors.
Once your community is taxonomically described, we move to functional characterization—revealing the metabolic potential and ecosystem roles within your sample. This can be achieved by mapping taxon-associated ecosystem functions or by predicting and annotating genes from assembled contigs. We translate taxonomic lists into actionable biological insights about what your microbial community is capable of doing.
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